A hole in the center of the stage allows light to pass from the light source beneath the stage through the slide specimen, which should be centered over the hole in the stage. Keep the stage dry at all times. Fluids can damage metal parts and also make it difficult to move a slide around on the stage.
https://www.youtube.com/watch?v=wGBHpWDWvjo
Why must the slide be kept in place?
It protects the microscope’s objective lens from contacting the specimen and vice versa; in oil immersion microscopy or water immersion microscopy the cover slip prevents contact between the immersion liquid and the specimen.
How can you prevent your slide from slipping on the stage?
How can you prevent your slide from slipping on the stage? Use the stage clips. Is the image seen through the microscope oriented the same way as the object on the stage of the microscope?
What is used to hold a slide in place on the stage?
Use the stage clips to hold the slide in place.
What is used to keep the glass slide in place and prevent it from moving or sleeping while placed on the stage?
Your microscope slide should be prepared by placing a coverslip or cover glass over the specimen. This will help protect the objective lenses if they touch the slide. Place the microscope slide on the stage and fasten it with the stage clips.
When you move the slide to the left on the stage what direction does the image appear to move?
A specimen that is right-side up and facing right on the microscope slide will appear upside-down and facing left when viewed through a microscope, and vice versa. Similarly, if the slide is moved left while looking through the microscope, it will appear to move right, and if moved down, it will seem to move up.
Should you focus by moving stage up or down?
Use the fine adjustment, if available, for fine focusing. If you have a microscope with a moving stage, then turn the coarse knob so the stage moves downward or away from the objective lens.
What is the function of the base on a microscope?
Base: The bottom of the microscope, used for support Illuminator: A steady light source (110 volts) used in place of a mirror. Stage: The flat platform where you place your slides. Stage clips hold the slides in place.
When you are placing your slide onto the stage for viewing the position of the stage should be?
THE OBJECTIVE SHOULD BE POSITIONED ABOUT 1/4″ – 3/8″ ABOVE THE SLIDE. 4. PLACE THE SLIDE ON THE MICROSCOPE STAGE SUCH THAT THE PORTION OF THE SLIDE YOU WANT TO VIEW IS UNDER THE OBJECTIVE.
What power must be in place when you first place a slide on the microscope stage?
What power must be in place when you first place a slide on the microscope stage? When focusing on a slide, ALWAYS start with either the 4X or 10X objective. Once you have the object in focus, then switch to the next higher power objective. Re-focus on the image and then switch to the next highest power.
What holds the slide and moves up and down?
Stage clips hold the slides in place. If your microscope has a mechanical stage, you will be able to move the slide around by turning two knobs. One moves it left and right, the other moves it up and down. Usually you will find 3 or 4 objective lenses on a microscope.
What are the function of the base and the arm of the microscope?
Base – It acts as microscopes support. It also carries microscopic illuminators. Arms – This is the part connecting the base and to the head and the eyepiece tube to the base of the microscope. It gives support to the head of the microscope and it is also used when carrying the microscope.
Do you place the slide under the clips in the mechanical stage?
If your microscope has stage clips, secure the slide under the stage clips. If your microscope has a mechanical stage, turn the control knobs (below one side of the stage) to position the specimen directly over the opening in the stage.
When carrying the microscope Why should one hand hold the bottom of the base?
Hold the microscope with one hand around the arm of the device, and the other hand under the base. This is the most secure way to hold and walk with the microscope. Avoid touching the lenses of the microscope. The oil and dirt on your fingers can scratch the glass.
What happens if you don’t use stage clips to secure your slide on the stage?
Terms in this set (59) use both stage clips to secure slides. Otherwise it will be very hard to make small adjustments in position while viewing specimens.
Why is it necessary for the specimen to be observed under the microscope must be thin?
For a typical microscope, meaning it is not an electron microscope or other expensive and complex technology, a specimen must be very thin to allow light to pass through it.
When carrying your microscope hold it by the base and the stage?
When carrying your microscope, hold it by the base and the metal support arm. Do not pick it up by the stage, as this can cause misalignment. When transporting it, use a microscope bag.
When you move the slide to the left in what direction does the letter E appear to move when you move it to the right up down?
The “e” is flipped and backwards. 3. While looking through the microscope, move the slide to the left, notice which way the letter “e” moved. Now move the slide to the right.
Under what objective do you see the largest area field of view from your slide?
lowest power objective
The field of view is largest on the lowest power objective. When you switch to a higher power, the field of view closes in towards the center. You will see more of an object on low power.
When positioning the slide under the lens What steps do you follow?
Steps on How to Use a Light Microscope
- Step 1: Connect the light microscope to a power source.
- Step 2: Turn the revolving nosepiece so the lowest objective lens is in position.
- Step 3: Mount your specimen onto the stage.
- Step 4: Use the metal clips to keep your slide in place.
Is the space between the bottom of the objective lens and the surface of the slide?
– WORKING DISTANCE is the space between the bottom of the objective lens and the surface of the slide. – FIELD OF VIEW is the area you see through the microscope.
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