Place the microscope slide on the stage (6) and fasten it with the stage clips. Look at the objective lens (3) and the stage from the side and turn the focus knob (4) so the stage moves upward. Move it up as far as it will go without letting the objective touch the coverslip.
Which part of the microscope is used to move the slide?
Coarse Adjustment Knob– The coarse adjustment knob located on the arm of the microscope moves the stage up and down to bring the specimen into focus. The gearing mechanism of the adjustment produces a large vertical movement of the stage with only a partial revolution of the knob.
Which direction do you move your slide?
How well do you know your Microscope?
| A | B |
|---|---|
| compound | microscope using 2 sets of lenses |
| left | direction image moves when you move the slide right |
| right | direction image moves when you move the slide left |
| towards | direction image moves when you move the slide away from you |
How do you move the slide around in your viewing field?
If you have a microscope with a moving stage, then turn the coarse knob so the stage moves downward or away from the objective lens. Move the microscope slide around so that the image is in the center of the field of view and readjust the mirror, illuminator or diaphragm for the clearest image.
What moves the slide left and right?
The stage clamp holds the microscope slide in place. Below the stage is a set of knobs called the STAGE ADJUSTMENT KNOBS. The top (larger) stage adjustment knob moves the stage vertically (towards you and away from you). The bottom (smaller) stage adjustment knob moves the stage horizontally (left/ right).
How do you adjust a microscope?
Use the coarse and fine focus control knobs to adjust the focus of the sample. When the sample is clearly visible, use only your left eye. Do NOT adjust the focus knobs. Instead, adjust the diopter on the left eyepiece until the sample comes clearly into view.
Where the slide specimen is placed?
Stage
Stage: The flat platform where the slide is placed. Stage clips: Metal clips that hold the slide in place. Stage height adjustment (Stage Control): These knobs move the stage left and right or up and down.
Which part of the microscope should you hold when lifting or moving the microscope?
The microscope arm connects the eyepiece tube to the base. This is the part you should hold when transporting a microscope.
How does the image move when the slide is moved to the right or left?
A specimen that is right-side up and facing right on the microscope slide will appear upside-down and facing left when viewed through a microscope, and vice versa. Similarly, if the slide is moved left while looking through the microscope, it will appear to move right, and if moved down, it will seem to move up.
How do you change the magnification of a microscope?
On a standard stereo microscope (not a common main objective stereo microscope) the objective lens is built into the microscope and the only way to change this magnification is by adding an auxiliary lens to the existing objective lens. These are typically available in increments of 0.5x, 0.75x and 1.5x magnification.
What happens when you move a slide up on a microscope?
Finally, remember! When you view a specimen through a microscope, you are viewing an image through multiple lenses. As a result, the image is upside down and back-to-front so when you move the slide to the right, the image moves to the left and vice versa!
How do you adjust the position of a slide on the stage?
Clip the slide into place with the stage clips. The cover slip on the slide must face up. Find the stage controls and make sure that, when they are turned, the slide moves smoothly left and right or up and down, depending on the knob.
What does a mirror do on a microscope?
Plane or concave mirror, placed on the microscope base and used to send light onto the specimen and into the microscope optics. The mirror is mounted on a swiveling support, adjusted to reflect natural light or light from an artificial source in the desired direction.
How do you adjust the slide when the specimen is out of the field of view to the top?
Slowly move the slide to center the specimen under the lens, if necessary. Do this by nudging it gently with your fingers or by turning the slide control knobs if you have a mechanical stage. Adjust the small fine focus knob until the specimen is clearly in focus. Then adjust the diaphragm to get the best lighting.
How do you focus a slide?
Viewing Prepared Slides
Start by rotating the objective lens to lowest power. Place a slide on the stage, label side up, with the coverslip centered. On LOW POWER ONLY, use the coarse focus knob to get the object into focus. If you cannot see anything, move the slide slightly while viewing and focusing.
How is magnification adjusted?
The magnification adjusts through combining the eyepiece and lenses of most microscopes. The standard eyepiece magnifies 10x. Check the objective lens of the microscope to determine the magnification, which is usually printed on the casing of the objective.
What does the coarse adjustment knob do on a microscope?
COARSE ADJUSTMENT KNOB — A rapid control which allows for quick focusing by moving the objective lens or stage up and down. It is used for initial focusing.
How do you handle a microscope?
Hold the microscope with one hand around the arm of the device, and the other hand under the base. This is the most secure way to hold and walk with the microscope. Avoid touching the lenses of the microscope. The oil and dirt on your fingers can scratch the glass.
What two parts of the microscope should you hold when carrying the microscope?
When carrying a compound microscope always take care to lift it by both the arm and base, simultaneously. There are two optical systems in a compound microscope: Eyepiece Lenses and Objective Lenses: Eyepiece or Ocular is what you look through at the top of the microscope.
What two parts do you place your hands while carrying a microscope?
When you move your microscope, you should always use two hands. Place one hand around the arm, lift the scope, then put your other hand under the base of the scope for support.
How do you focus a slide using the high power objective?
To focus at the highest power, rotate the nosepiece to the objective lens (40X) while looking at it from the side. If you do not look at it from the side you may crack your slide or the objective lens. Remove object from stage and make sure stage is clean. Turn off microscope light and unplug microscope.
Contents