Locate the dirt within the optics of your microscope If you see a spot of dirt on your image when viewing at all objective powers, the dirt is likely to be on the eyepiece lens. However, if you only see dirt at one power, it is likely to be on the objective lens. To check this, turn the eyepiece lens if you can.
What will you use to clean the lenses of the microscope Why would you use this?
To clean microscope eyepiece lenses, breathe condensation onto them and then wipe them with lens tissue. Kim-wipes are made by Kleenex and generally will work well. For stubborn spots, wipe the surface with tissue moistened with 95% alcohol.
What device is used to test the optics of the high power lens?
With a dioptometer, also referred to as a diopter telescope, in hand, you can measure the focal power of a lens in a few simple steps.
What regulates the amount of light in the microscope Why do you need to regulate it?
The condenser serves two purposes; it regulates the amount of light reaching the specimen and it focuses the light coming from the light source. As the magnification of the objective lens increases, more light is needed. The iris diaphragm (located in the condenser), regulates the amount of light reaching the specimen.
Why is it needed for you to know the different parts and functions of the microscope?
Knowing the names of the different parts of a microscope is important because it helps you communicate clearly with students or colleagues. Remembering the different parts is easy if you keep a labeled diagram with you and make sure you always use the proper terms when referring to the different parts of a microscope.
How do we clean the optical system of the microscope?
To clean the eyepiece lens of your microscope, breathe onto the eyepiece lens and then wipe with lens tissue. For dirt that is difficult to remove, add ethanol (methanol in extreme cases) to a cotton swab, wipe the surface and then dry with a dry swab.
How does the dissecting microscope work?
A stereo or a dissecting microscope uses reflected light from the object. It magnifies at a low power hence ideal for amplifying opaque objects. Since it uses light that naturally reflects from the specimen, it is helpful to examine solid or thick samples.
What adjustment knob is used with high power?
fine adjustment knob
Use ONLY the fine adjustment knob to focus on high power. NEVER USE THE COARSE FOCUS KNOB ON HIGH POWER! The high power lens should be very close to your slide when in proper focus. If you turn the coarse adjustment knob while on high power, the objective could easily break your slide.
How is the beam focused in a light microscope?
Light microscopes use a condenser above the light source in order to focus and direct the light up through the specimen (in a small beam of light) and into the eyepieces.
What regulates the amount of light in the microscope?
Iris Diaphragm controls the amount of light reaching the specimen. It is located above the condenser and below the stage. Most high quality microscopes include an Abbe condenser with an iris diaphragm.
How do you control the amount of light that enters a microscope?
The condenser is equipped with an iris diaphragm, a shutter controlled by a lever that is used to regulate the amount of light entering the lens system. Above the stage and attached to the arm of the microscope is the body tube. This structure houses the lens system that magnifies the specimen.
What regulates the amount of light shining through the microscope?
IRIS DIAPHRAGM CONTROL — A lever (or rotating disk) that adjusts the amount of light illuminating the slide.
What is located on the side of the frame used to adjust the focus of the microscope?
Coarse
Coarse and Fine Adjustment knob(s) – Located on the side of the frame, below the stage, allow the observer to adjust the focus of the microscope.
Where is the safest place to hold or carry a microscope?
Hold the microscope with one hand around the arm of the device, and the other hand under the base. This is the most secure way to hold and walk with the microscope. Avoid touching the lenses of the microscope. The oil and dirt on your fingers can scratch the glass.
Which of the following parts of the microscope holds the slide in place?
stage clips
Use the stage clips to hold the slide in place.
What happens to a microscope if place at the edge?
Answer: When the object on your slide is in focus for each objective, the distance between the slide and the objective lens, the working distance, decreases as the objective magnification increases.
WHAT IS lens paper?
Definition of lens paper
: a soft nonabrasive lintless tissue paper used for wiping and wrapping lenses.
Which objective must be in place when you put the microscope away?
Always place the 4X objective over the stage and be sure the stage is at its lowest position before putting the microscope away.
What are the cleaning materials that are needed for proper care of the microscope?
Keep area clean
Keep the stage clean with lens paper to remove dust and particles, you can use a little alcohol to remove oil and other dirt if needed. A small soft brush or canned air can be used to dust off the microscope – use carefully. Do not prepare or stain samples next to the microscope.
How do you clean the lenses of your microscope quizlet?
Terms in this set (13)
- turn the light control all the way down.
- turn the microscope off.
- rotate the nosepiece to the 4x objective.
- drop the stage all the way down using the coarse focus knob.
- remove your microscope slide from the stage.
- using a dry piece of lens paper, wipe the 40x objective first in a circular motion.
How do you prepare the lens to clean microscope?
To remove other oily substances, we recommend using the detergent called Wisk and prepare a solution of 1 part Wisk to 100 parts water. Try not to remove the lenses from your microscope unless absolutely necessary and remember to keep it covered as dust is the number one enemy!
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