The purpose of the condenser lens is to focus the light onto the specimen. Used to vary the intensity and size of the cone of light that is projected upward into the slide.
What is the function of the condenser lens?
A condenser is an optical lens which renders a divergent beam from a point source into a parallel or converging beam to illuminate an object. Condensers are an essential part of any imaging device, such as microscopes, enlargers, slide projectors, and telescopes.
What is the purpose of the condenser microscope quizlet?
What is the purpose of the condenser? to allow the maximum amount of light to enter the specimen.
What does condenser focus do in a microscope?
Condenser Focusing Knob – This control is used to precisely adjust the vertical height of the condenser. Condenser Lens – This lens system is located immediately under the stage and focuses the light on the specimen.
What are the functions of condenser and diaphragm?
Many modern course microscopes are equipped with a condenser and an associated condenser diaphragm. The purpose of the condenser is to concentrate the light onto the specimen, its diaphragm regulates resolution, contrast and depth of field.
Which objective lens is most commonly used to observe bacteria quizlet?
Explain your answer. The 100x lens is used most commonly b/c it allows the shapes and forms of microbes to be observed.
What is the purpose of the immersion oil?
Immersion oil increases the resolving power of the microscope by replacing the air gap between the immersion objective lens and cover glass with a high refractive index medium and reducing light refraction.
What is involved in making a smear quizlet?
A sample of a bacterial colony must be mixed into water or saline. Using a pipette, apply a small drop of the bacterial fluid to a clean glass slide. Smear the fluid evenly across the surface of the slide with the edge of a cover slip. Allow slide to air dry.
What is the difference between condenser lens and objective lens?
The condenser lens defines the size of the electron beam (which defines the resolution), while the main role of the objective lens is to focus the beam onto the sample.
What is the function of an ocular lens in a microscope?
The eyepiece, or ocular lens, is the part of the microscope that magnifies the image produced by the microscope’s objective so that it can be seen by the human eye.
How do the functions of the sub stage condenser and the iris diaphragm within the condenser differ?
The iris diaphragm controls the amount of light passing through the slide or specimen, while the substage condenser focuses a cone of light on the slice or specimen.
What position should the condenser be in when working at the 100X objective?
For work with the high-dry (40x) and oil-immersion objectives (100x), however, the diaphragm should be closed slowly while looking at a sharply focused section until the level of illumination is just slightly reduced. This is the setting of the condenser diaphragm for optimum contrast and resolution.
What is the purpose of the immersion oil quizlet?
In light microscopy, oil immersion is a technique used to increase the resolution of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.
What objective lens is used for oil immersion?
100x objective lens
Before using immersion oil, make sure that your 100x objective lens is made for use with immersion oil. Begin by focusing your sample using the 40x objective lens. Rotate the objective lens part way between the 40x and 100x lens so you can reach the cover slip on your slide.
What is the purpose of immersion oil when using the 100x lens?
Oil immersion is the technique of using a drop of oil to wet the top of the specimen or slide cover and the front of the objective lens. This effectively immerses or bathes the light path between the lens and object viewed, allowing finer details to be seen.
Why do you use immersion oil with the 100x objective lens?
The 100x lens is immersed in a drop of oil placed on the slide in order to eliminate any air gaps and lossof light due to refraction (bending of the light) as the light passes from glass (slide) → air → glass (objective lens).
What is the purpose of a smear?
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium.
What is smear in microbiology quizlet?
A dried preparation of bacterial cells on a glass slide is called a. smear.
What is the purpose of applying a smear and fix?
Smears are ready to heat fix when they are completely air-dried. When are smears ready to heat-fix? The purpose of heat-fixing a smear is to make the cells stick to the slide.
What is the function of the ocular lens quizlet?
The function of the ocular lens (eye piece) is to magnify the image produced by the objective lens. The ocular lens produces a virtual image that appears below or within the microscope. Define resolving power.
What is the function of objective lens and ocular lens?
The objective, located closest to the object, relays a real image of the object to the eyepiece. This part of the microscope is needed to produce the base magnification. The eyepiece, located closest to the eye or sensor, projects and magnifies this real image and yields a virtual image of the object.
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